ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak patikan kebo (Euphorbia hirta L.) terhadap daya tetas telur ikan patin (Pangasius sp.). Penelitian dilakukan pada bulan Agustus 2014 di UPTD-BAT (Unit Pelaksana Teknis Dinas – Budidaya Air Tawar) Jantho Baru, dan Laboratorium Kimia, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Syiah Kuala. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) dengan 4 taraf perlakuan A(0 ppm); B(20 ppm); C(60 ppm); D(100 ppm) dan 5 ulangan. Sampel yang digunakan adalah telur ikan patin yang terserang jamur Saprolegnia sp. sebanyak 100 butir per wadah/ulangan. Hasil uji ANSIRA menunjukkan bahwa penambahan ekstrak patikan kebo berpengaruh sangat signifikan terhadap penetasan telur ikan patin pada taraf uji 1%. Hasil penelitian menunjukkan pada perlakuan A (0 ppm) telur ikan patin yang menetas hanya 42,4%. Berdasarkan uji lanjut BNTdidapatkan bahwa perlakuanC (60 ppm) merupakan perlakuan dengan daya tetas tertinggi sebesar 92,6 %. Kata kunci: Ikan patin, penetasan telur, ekstrak patikan kebo. iiABSTRACT This research aimed to find out the influence of Euphorbia hirta L. extract on hatching rate of Pangasius sp. eggs. The research was conducted on August 2014 at the UPTD- BAT (Unit Pelaksana Teknis Dinas –Budidaya Air Tawar) Jantho Baru, and Chemistry Laboratory, Teacher Training and Education Faculty, Syiah Kuala University. This research used a Completely Randomized Design (CRD) with four treatments of Euphorbia hirta L. extract concentration A(0 ppm); B(20 ppm); C(60 ppm); D(100 ppm) and five replications. This research usedPangasius sp. eggs as sample attacked by Saprolegnia sp., it was distributed 100 eggs of compartment. The ANOVA test showed that the concentration of Euphorbia hirta L gave the significant effect on hatching rate of Pangasius sp. eggs at test level of 1%. The result showed that at A (0 ppm) , the hatching rate of Pangasius sp. was 42,4%. The least significant different test showed that the higher hatching rate performance was found at C (60 ppm) of 96,2%. Keywords: Pangasius sp., HatchingRate, Euphorbia hirtaL.