ABSTRAK Telah dilakukan penelitian tentang uji aktivitas antimikrobial ekstrak n-heksan resin jernang dan identifikasi kandungan senyawa kimia menggunakan spektroskopi GC-MS. Hasil karakterisasi ekstrak menunjukkan bahwa ekstrak n-heksan resin jernang Bener Meriah mengandung kadar air 3,9%, kadar abu total 7,53%, kadar sari larut air 3,33% dan kandungan kadar sari larut etanol 85,83%. Uji fitokimia menunjukkan bahwa ekstrak n-heksan resin jernang mengandung senyawa flavonoid. Hasil uji aktivitas ditentukan dengan metode sumuran difusi agar menggunakan teknik cawan tuang memperlihatkan bahwa ekstrak n-heksan resin jernang pada range konsentrasi 5-15% mempunyai aktivitas antimikrobial dengan diameter daya hambat berkisar antara 9,0 - 12,3 mm terhadap Staphylococcus aureus ATCC 25923 dan 16,0 - 21,3 mm terhadap Escherichia coli ATCC 25922, serta 8,6 – 13,0 mm terhadap Candida albicans ATCC 10231. Hasil analisis spektroskopi FTIR terhadap ekstrak n-heksan resin jernang, menunjukkan adanya serapan –C-O-C (eter) pada bilangan gelombang 1263.38 cm-1, serapan siklik aromatik (-C=C) pada bilangan gelombang 1605.01 cm-1 dan serapan gugus hidroksil (-OH) pada bilangan gelombang 3361.02 cm-1. Hasil analisis spektroskopi GC-MS memperlihatkan adanya senyawa 2H-7ol-3,4-dihydro-5-methoxy-6-methyl-2-phenyl (C17H 18O3) yang merupakan senyawa penciri jernang pada RT 26 dengan nilai m/z yaitu 270 g/mol. Kata kunci: Jernang (Daemonorops draco Blume), ekstrak n-heksan resin jernang, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Candida albicans 10231. ABSTRACT The research of the antimicrobial activity and identification of chemical coumpound of n-hexane dragon blood’s exctract by GC-MS spectroscopy has been conducted. The characterization of extract showed that n-heksan dragon blood’s extract comprise water content of 3,9%, total ash content of 7,53%, water soluble extract content of 3,33%, and etanolic soluble extract content of 85,83%. The phythochemicals assays showed that the extract was contain flavonoid. The antimicrobial activity the extract was determined using well diffusion method with pour plate technique showed that the extract at concentration range of 5-15% exhibited antimicrobial activity with a diameter of inhibition at range between 9-12,3 mm againt Stahpylococcus aureus, 16-21,3 mm againt Escherichia coli, and 8,6-13 mm againt Candida albicans. The FTIR spectroscopy analysis showed that the spectra at 1263,38 cm-1 is represented the –C-OC (eter) vibration spectra at 1605,01 cm-1 is represented the cyclic aromatic (C=C) vibration and the spectra at 3361,02 cm-1 is represented the –OH (hydroxyl) vibration. The GC-MS analysis indicated that the extract contain 2H-7ol-3,4-dihydro-5-methoxy-6-methyl-2-phenyl (C17H 18O3) at Rt 26 with mass ration m/z is 270 g/mol. Keywords: Dragons blood (Daemonorops draco Blume), dragon blood n-heksan extract, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Candida albicans 10231.