ANALISIS HORMON STRES SECARA NON - INVASIF PADA GAJAH SUMATERA (Elephas maximus sumatranus) DI PUSAT KONSERVASI GAJAH SAREE - ACEH BESAR ABSTRAK Pengukuran hormon stres secara non-invasif lebih efektif untuk diterapkan pada satwa liar seperti gajah sumatera. Penelitian ini bertujuan untuk mengukur konsentrasi metabolit hormon kortisol secara non-invasif dan korelasinya dengan faktor lingkungan yaitu suhu dan curah hujan pada gajah sumatera di Pusat Konservasi Gajah (PKG) Saree, Aceh Besar. Sampel feses dikoleksi dari lima ekor gajah sumatera selama lima minggu (1 sampel/ekor/minggu). Data suhu lingkungan, curah hujan, dan suhu rektal (tubuh) diukur bersamaan dengan koleksi sampel feses. Feses diekstraksi dan diukur konsentrasi metabolit kortisol menggunakan metode enzyme-linked immunosorbent assay (ELISA). Data yang diperoleh dianalisis menggunakan uji korelasi untuk melihat hubungan antara konsentrasi metabolit kortisol dengan parameter suhu lingkungan, curah hujan, dan suhu rektal (tubuh). Hasil penelitian diperoleh rataan (±SD) konsentrasi metabolit hormon kortisol pada sampel feses gajah sumatera pada minggu 1-5 berturut-turut sebesar 138,46±24,26 ng/g, 79,57±14,91 ng/g, 98,21±9,53 ng/g, 133,31±12,42 ng/g dan 108,67±25,82 ng/g. Hasil analisis statistik menunjukkan adanya peningkatan konsentrasi metabolit kortisol yang signifikan seiring dengan peningkatan suhu lingkungan aktual dan suhu tubuh (P˂0,05). Kesimpulan, konsentrasi metabolit hormon kortisol pada sampel feses gajah sumatera mengalami fluktuasi dan berkorelasi dengan suhu lingkungan aktual dan suhu tubuh. Kata kunci: Gajah Sumatera, Metode Non-invasif, Kortisol, Stres NON-INVASIVE STRESS HORMONE ANALYSIS IN SUMATRAN ELEPHANT (Elephas maximus sumatranus) AT THE ELEPHANT CONSERVATION CENTER SAREE - ACEH BESAR ABSTRACT Non-invasive measurement of stress hormones is more effective to be applied to wildlife such as the Sumatran elephant. This study aims to measure the concentration of cortisol hormone metabolites in a non-invasive manner and its correlation with environmental factors, namely temperature and rainfall on a Sumatran elephant at the Elephant Conservation Center Saree, Aceh Besar. Samples were collected from five Sumatran elephants for five weeks (1 sample / head / week). Environmental temperature data, rainfall, and rectal temperature (body) were measured together with the collection of faecal samples. Fecal samples were extracted and the concentration of cortisol metabolites was measured using the enzyme-linked immunosorbent assay (ELISA) method. The data obtained were analyzed using a correlation test to determine the relationship between the concentration of cortisol metabolites and parameters of environmental temperature, rainfall, and rectal temperature (body). The mean (± SD) of cortisol metabolites concentration in Sumatran elephant faecal samples in weeks 1-5 were 138.46 ± 24.26 ng/g, 79.57 ± 14.91 ng/g, 98,. 21 ± 9.53 ng/g, 133.31 ± 12.42 ng/g and 108.67 ± 25.82 ng/g. Respectively the results of statistical analysis showed that increase of cortisol metabolites concentration was significant related with the increase of actual environmental temperature and body temperature (P˂0.05). In conclusion, the concentration of cortisol metabolites in the fecal of Sumatran elephants has fluctuated and correlated with the actual environmental temperature and body temperature. Keywords: Sumatran Elephant, Non-invasive method, Cortisol, Stress.