Bakteri endofit merupakan mikroorganisme menguntungkan yang berinteraksi dengan tanaman inang tanpa menyebabkan gangguan atau kerusakan pada tanaman. Penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit dari jaringan akar tanaman padi (Oryza sativa L.), yang berpotensi menghasilkan hormon IAA, mengidentifikasi bakteri endofit secara morfologis dan fisiologis, serta analisis gen 16S rRNA isolat terpilih. Berdasarkan hasil penelitian diperoleh bakteri endofit sebanyak enam isolat. Bakteri endofit memiliki keragaman morfologi tinggi dan kemampuan untuk menghasilkan hormon IAA yang beragam. Konsentrasi IAA tertinggi (425 ppm) diperoleh isolat dari EAP3. Berdasarkan uji biokimia, EAP3 memiliki kemiripan 60% dengan Enterobacter asburiae. Analisis gen 16S rRNA menunjukkan bahwa EAP3 memiliki kemiripan tertinggi dengan Enterobacter asburiae strain U4 sebesar 99%. Data penelitian ini dianggap sebagai informasi baru tentang potensi bakteri endofit dari akar tanaman padi (Oryza sativa L.) yang mampu menghasilkan hormon IAA. Kata kunci: Bakteri endofit, padi (Oryza sativa L.), hormon IAA, EAP3 Endophytic bacteria are beneficial microorganisms that interact with host plants without causing any interference or damage to plants. This research aimed to obtain endophytic bacteria isolated from the root tissue of rice plants (Oryza sativa L.) which have potential to produce IAA hormones, identify the endophytic bacteria in morphologically and physiologically, and analysis of the selected isolate 16S rRNA genes. Based on the results of this research, there were six endophytic bacteria isolates obtained. They have high morphological diversity and differen ability producing IAA hormones. The highest concentration of IAA (425 ppm) was obtained isolates from EAP3. Based on the biochemical test, EAP3 had 60% similarity with Enterobacter asburiae. Analysis of the 16S rRNA gene showed that EAP3 had the highest similarity with Enterobacter asburiae strain U4 by 99%. This research data is considered as new information about the potential of endophytic bacteria from the roots of rice plants (Oryza sativa L.) which is capable of producing IAA hormones. Keywords: Endophytic bacteria, rice (Oryza sativa L.), IAA hormone, EAP3