ABSTRAK Salah satu bakteri endofit yang berpotensi menghasilkan senyawa bioaktif yang sama dengan tanaman inangnya berasal dari kelompok aktinobakteri.Penelitian ini bertujuan untuk mengisolasi aktinobakteri endofit dari tanaman belimbing wuluh (Averrhoa bilimbi L.), melihat keragaman aktinobakteri endofit, dan mengetahui kemampuan aktinobakteri endofit memproduksi inhibitor lipase pankreas dan menentukan kandungan senyawa metabolit sekunder. Isolasi aktinobakteri endofit menggunakan media Humic Acid Vitamin B Agar (HV). Aktivitas inhibitor lipase pankreas diuji menggunakan lipase (L3126 Sigma) diukur menggunakan spektrofotometer. Sebanyak enam isolat berhasil diisolasi. Tiga isolat aktinobakteri endofit belimbing wuluh (Averrhoa bilimbi L.) menghasilkan aktivitas inhibitor lipase pankreas yaitu isolat AEDB2, AEAB1, dan AEAB4. Aktivitas tertinggi dihasilkan oleh isolat AEDB2 dengan nilai inhibitor 78%. Hasil fitokimia isolat AEDB2 terdeteksi mengandung senyawa terpenoid, steroid, saponin, dan tanin. Kata kunci: Aktinobakteri endofit, belimbing wuluh (Averrhoa bilimbi L.), inhibitorlipase pankreas. ABSTRACT Actinobacteria are endophytic bacteria that have potential to produce the similar bioactive compounds as their host plants. The objectives of the study were to isolate endophytic actinobacteria from the starfruit (Averrhoa bilimbi L.) plant, to observe the diversity of endophytic actinobacteria, to find out the ability of endophytic actinobacteria in producing pancreatic lipase inhibitors, and determine the content of secondary metabolites. Isolation of endophytic actinobacteria was conducted using the Humic Acid Vitamin B Agar (HV) media. The pancreatic lipase inhibitor activity was tested using lipase (L3126 Sigma) and measured using a spectrophotometer. A total of six isolates were successfully isolated. Three endophytic actinobacterial isolates of starfruit (Averrhoa bilimbi L.) produced pancreatic lipase inhibitors activity, namely AEDB2, AEAB1, and AEAB4 isolates. The highest activity was produced by AEDB2 isolates with inhibitor values of 78%. The phytochemical results of AEDB2 isolates were detected to contain terpenoids, steroids, saponins, and tannins. Keywords: Endophytic actinobacteria, starfruit (Averrhoa bilimbi L.), pancreatic lipase inhibitors.