Cellular phone or mobile phone is wireless communication device that is used by all ages around the world because it has many benefits. However cell phones can be harmful and have many negative impacts on health, one of them is headache that many cell phone users complained about. This research aims to know the effect of cell phone usage on headache in preclinical students of Medical Faculty Syiah Kuala University. This research is an observational analytic with cross-sectional approach. The sampling technique is stratified random sampling with total amount of sample is 231 subjects. To collect data this reasearch used a validated cell phone usage questionnaire and Visual Analog Scale (VAS) to assess the scale of headache from cell phone users. Data were analyzed using the Spearman Rank test with 95% as the confidence level. The result of this research showed that from 231 respondents there are 104 people (45%) has complained of mild headache and 23 people (10%) moderate headache. From this research showed that there is a significant relation between cell phone usage and headache (p=0,000) and there is a significant relation too between the time of staring at screen of cell phone and headache (p=0,000), but there is no significant relation from type of cell phone cards frequency or called SIM cards (p=0,343), number of calls per day (p=0,369), duration of calls per day (p=0,092), and visibility of cell phone screen (p=0,403). The conclusion from this research is there is significant relation between cell phone usage on headache in preclinical students Medical Faculty Syiah Kuala University. Keywords: cell phone, headache, students Medical Faculty Syiah Kuala University