ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak metanol daun pandan wangi (Pandanus amaryllifolius Roxb.) terhadap kadar HDL dan LDL darah tikus (Rattusnovergicus) hiperkolesterolemia. Penelitian menggunakan rancangan acak lengkap (RAL) yang terdiri dari 5 perlakuan dan 4 ulangan. Pemberian 0 ml/g bb (K-), pemberian 4 ml/200 g bb pakan hiperkolesterolemia (K+), pemberian 4 ml/200 g bb pakan hiperkolesterolemia dan ekstrak daun pandan wangidosis 20% (P1), 40% (P2), dan 60% (P3). Ekstrak daun pandan wangi diberikan selama satu kali sehari selama 14 hari secara oral, setelah 6 hari pemberian pakan hiperkolesterolemia. Parameter yang diamatiadalahkadar HDL dankadarLDL darahtikus(R.novergicus) jantanhiperkolesterolemia sebelum dan sesudah diberikan ekstrak daun pandan wangi. Analisis data menggunakan analisis varians (ANAVA) yang dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun pandan wangi dapat menurunkankadarLDLdosis 40% (P2) 48,750danmeningkatkan kadar HDLdarahtikuskembalidalamkeadaan normal dengandosis 20% (P1) 35,000 darah tikus hiperkolesterolemia. Kata kunci:High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL), Rattus novergicus, Pandanus amaryllifoliusRoxb. ABSTRACT The objective of this study was to determine the effect of methanol extract of fragrant pandan leaf (Pandanus amaryllifolius Roxb.) to the levels of HDL and LDL in hypercholesterolemia rat (Rattusnovergicus) blood. The experiment used a complete randomized design consisting of 5 treatments and 4 replications by giving 0 ml/g bb (P0), 4 ml/200 g bb hypercholesterolemia feed (P1), 4 ml/200 g bb hypercholesterolemia feed and the extract of fragrant pandan leaf dose 20% (P2), 40% (P3), and60% (P4).The fragrant pandan leaf extract was given once a day for 14 days orally after giving hypercholesterolemia feed for 6 days. The parameters measured were the levels of HDL and LDL in hypercholesterolemia male rat (R.novergicus) blood before and after giving extract of fragrant pandan leaf (P. amaryllifolius Roxb.). The data was analyzed by using a analysis of variance(ANOVA) followed by the Tukey test. The results suggest that giving fragrant pandanleaf can lower LDL levels dose 40% (P2) 48,750 and increase HDL levels dose 20% (P1) 35,000 pandan left extract in hypercholesterolemia rat normal blood. Keywords: High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL), Rattus novergicus, Pandanus amaryllifoliusRoxb.