ABSTRAK Penelitian ini bertujuan untuk mengetahui pertumbuhan benih ikan kakap putih (Lates calcarifer) yang dipelihara di air bersalinitas rendah. Penelitian ini telah dilakukan pada bulan Juni 2013 di Laboratorium Pakan Buatan BBAP Ujung Batee. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak lengkap (RAL) dengan 5 (lima) perlakuan (0 ppt; 5 ppt; 10 ppt; 15 ppt; 31 ppt) dan 3 (tiga) ulangan. Data di olah dengan menggunakan Analysis of Variance (ANOVA). Hasil penelitian menunjukkan bahwa benih ikan kakap putih yang dipelihara pada salinitas rendah tidak berbeda nyata serta tidak berpengaruh terhadap pertumbuhan. Namun dari hasil analisis varian dapat dilihat bahwa nilai tertinggi terdapat pada perlakuan D salinitas 15 ppt pada pertumbuhan berat relatif dan panjang mutlak. Kata kunci : ikan kakap putih, salinitas, pertumbuhan, Lates calcarifer. ABSTRACT The objective of this research was to find out the growth of Barramundi (Later calcarifer) which was cultured in low salinity water. The research was conducted on June 2013 at laboratory of artificial feed, the Brackish Water Aquaculture Development Center, Ujong Batee. The research was designed using Completely Randomized Design with five treatments of salinity (0; 5; 10; 15; and 31 ppt) and three replications. The data was analyzed using Analysis of Variance (ANOVA). The result showed that the cultured fingerling of Barramundi in low salinity water found no significant effect on the its growth. But result of variance analysis was obtained that the highest valve was at the treatment of D (salinity of 15 ppt) specially for the relative growth and length. Keywords: barramundi, salinity, growth, Lates calcarifer.