ABSTRAK Resistensi antibiotik terhadap bakteri mendorong dilakukannya berbagai penelitian untuk mendapatkan bahan baku obat yang berasal dari alam sebagai antibakteri. Penelitian ini menguji aktivitas antibakteri ekstrak n-heksana, etil asetat, dan metanol daun peria laut (Colubrina asiatica (L.) Brongn) terhadap bakteri Methicillin Resistants Staphylococcus aureus (MRSA). Ekstraksi daun peria laut dilakukan dengan metode maserasi bertingkat menggunakan ekstrak n-heksana, etil asetat, dan metanol. Terhadap masing-masing ekstrak dilakukan uji fitokimia dan karakterisasi ekstrak. Uji aktivitas antibakteri dilakukan menggunakan metode Kirby-Bauer dengan variasi konsentrasi 5, 10, 15, dan 20%. Hasil uji fitokimia menunjukkan bahwa ekstrak n-heksana tidak memberikan hasil positif terhadap golongan senyawa alkaloid, saponin, tanin, flavonoid, dan steroid/triterpenoid. Ekstrak etil asetat mengandung senyawa saponin dan steroid. Ekstrak metanol mengandung senyawa tanin, flavonoid, saponin dan steroid. Hasil karakteristik ekstrak n-heksana, etil asetat dan metanol menunjukkan kadar air sebesar 6,70; 32,11; dan 16,52%, kadar sari larut air sebesar 4,31; 2,65; dan 16,52%, kadar sari larut etanol sebesar 8,28; 56; dan 54,52%, kadar abu total sebesar 0,14; 6,37; dan 4,83%. Hasil uji aktivitas antibakteri menunjukkan bahwa ekstrak n-heksana dan metanol tidak memiliki zona hambat, sedangkan ekstrak etil asetat menunjukkan zona hambat terbesar pada konsentrasi 20% dengan ratarata diameter zona hambat 10,49 mm. Kata kunci: Colubrina asiatica (L.) Brongn, Methicillin Resistants Staphylococcus aureus, dan metode Kirby-Bauer. ABSTRACT Antibiotic resistence on Bacterial encourages varicus research to find raw material drugs from nature as antibacterial. Antibacterial activity test of n-hexane, ethyl acetate, and methanol extract of peria laut leaves was conducted againts Metichillin Resistant Staphylococcus aureus (MRSA). Extraction was accomplished using solvent multistep maceration method by n-hexana, ethyl acetate, and methanol. The charactetization of extract and phytochemical test were evaluated for peria laut leaves extract. Antibacterial activity was determined by Kirby-Bauer method with variation of concentration 5, 10, 15, and 20%. Phytochemical test results showed that n-hexane extract was not give positive result on alkaloid, saponin, tannin, flavonoid and steroid/triterpenoid. Ethyl acetate extract contains saponin and steroid compound. Methanol extract contains tannin, flavonoid, and steroid. Characterization extract n-hexane, ethyl acetate, and methanol extract showed that water content 6.70; 32.11; and 16.52%, water soluble extract were 4.31; 2.65; and 54.52%, ethanol soluble extract 8.28; 56.26; and 54.53%, and total ash value 0.14; 6.37; and 4.83%. The results of antibacterial activity test showed that n-hexane and methanol extract were not present inhibition zone, however ethyl acetate extract showed that the largest inhibition zone on 20% concentration with average diameter was 10,49 mm. Keywords: Colubrina asiatica (L.) Brongn, Metichillin Resistant Staphylococcus aureus, and Kirby-Bauer method.