Abstrak. Penelitian mengenai struktur mikroskopis kelenjar perineal musang pandan (Paradoxurus hermaphroditus) dilaksanakan daribulan Juni sampai November 2013. Penelitian dilakukan di Laboratorium Mikroteknik FMIPAUniversitas Syiah Kuala, Banda Aceh. Rancangan eksploratif dan metode purposive sampling digunakan dalam penelitian ini. Hewan yang digunakan adalah tiga ekor P. hermaphroditus jantan. Pembuatan sediaan histologis kelenjar perineal P. hermaphroditus menggunakan metode parafin dan pewarnaan hematoksilin dan eosin. Hasil penelitian menunjukkan bahwa kelenjar perineal P. hermaphroditus berjumlah sepasang, terletak di bagian kiri dan kanan perineum. Masing-masing kelenjar perineal tersebut terdiri atas dua tipe, yaitu holokrin dan apokrin. Kelenjar tipe holokrin dan apokrin terletak secara berselang-seling mengelilingi lumen kelenjar perineal. Struktur kelenjar tipe holokrin adalah tubuloalveolar kompleks sedangkan tipe apokrin adalah tubuler simpleks bergelung. Sel mioepitel terletak di bagian basal dari asinus maupun duktus pada masing-masing tipe kelenjar. Kelenjar tipe holokrin dan apokrin dikelilingi oleh jaringan ikat longgar. Permukaan luar kelenjar perineal dilapisi oleh kapsula yang terdiri atas jaringan ikat sedangkan bagian dalam oleh epitel pipih berlapis. Kata kunci: P. hermaphroditus,kelenjar perineal,hematoksilin – eosin, holokrin, apokrin Abstract. This research about the microscopic structure of the perineal glands of common palm civet (Paradoxurus hermaphroditus) has been done from June to November 2013. The research was conducted in the Microtechnic Laboratoryof the Faculty of Mathematics and Natural Sciences of Syiah Kuala University, Banda Aceh. Explorative design and purposive sampling were applied of the research, consisted of three male P. hermaphroditus. Paraffin method and hematoxylen – eosin staining were applied of the perineal gland process in histological technic. The result showed that P. hermaphroditushad a patch of the perineal glands devided into right and left halves of perineum. Each of halves consisted of two types of glands, namely holocrine and apocrine. Apocrine and holocrine glands located criss-cross around the perineal gland lumen. Holocrine gland shape is complex tubuloalveolar while apocrine shape is coiled simplex tubular. Myoepithelial cells located in the basal of both the acini and ducts of apocrine and holocrine. Loose connective tissue surrounded to holocrine and apocrine glands. The outer surface of the perineal glands is covered by a capsule, consisting of connective tissue, while the inside is lined by stratified squamous epithelium. Key words: P. hermaphroditus, perineal gland, hematoxylen – eosin, holocrine, apocrine