ABSTRACT Membrane adsorption purolit has been made from polymer chitosan and particulate purolite with composition 3%(w/v) chitosan, 15%(v/v) dimethyl formamide and 60% adsorbent in this research. The characterization result of membrane indicates that the membrane have 42.84% of porosity, 74.95% of swelling degree and 258.4 L/m2h water flux. Samples of crude biodiesel contains glycerol amounted to 0.61%-b. The membrane adsorption test value of glycerol in biodiesel is 63.93%, glycerol that contained in biodiesel has been adsorbed for 60 minutes by the membrane and glycerol remaining is 0.23%-b. The results indicated that the glycerol content meets quality standards. Regeneration of membrane by methanol can reduce the value of glycerol until 54.09% and by ethanol is 18.03%. These results indicated that methanol solvent is better than ethanol solvent in regenerating membrane. The adsorption membrane that has been regenerated can be reused to reduce the value of glycerol in biodiesel. Key words: biodiesel, chitosan, purolit, membrane, adsorption, glycerol.