ABSTRAK Media buatan kultur in vitro merupakan media alternatif pengganti media Murashige dan Skoog (MS) yang umum digunakan seperti Growmore. Penambahan air kelapa dalam media dapat mempengaruhi pertumbuhan tanaman, sehingga perlu dilakukan penelitian tentang pengaruh penambahan beberapa konsentrasi air kelapa dalam media buatan terhadap pertumbuhan krisan (Dendranthema grandiflora). Penelitian ini dilaksanakan di Laboratorium Biologi Sel dan Molekuler Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Syiah Kuala, Banda Aceh dari bulan April sampai Juli 2013. Penelitian ini menggunakan Rancangan Acak Kelompok Sub Sampling dengan media sebagai kelompok dan air kelapa sebagai perlakuan. Pengamatan dilakukan mulai dari penanaman eksplan sampai satu bulan. Hasil penelitian menunjukkan tidak ada perbedaan nyata antara penggunaan media MS dan media buatan dengan beberapa konsentrasi air kelapa terhadap inisiasi tunas, akar, serta pertumbuhan daun. Perlakuan K1 (2,5% air kelapa dalam media) menunjukkan perbedaan nyata dengan K0 (0% air kelapa dalam media) terhadap tinggi batang krisan. Pertumbuhan panjang akar krisan terbaik ditunjukkan pada media A (MS) yaitu 4,8125 cm, sama halnya dengan pertumbuhan planlet (10,5042 cm), akan tetapi pertumbuhan planlet tidak berbeda nyata dengan pertumbuhan pada media D (Growmore 1 gram/L) yaitu 9,1750 cm. Kata kunci: Dendranthema grandiflora, air kelapa, media MS, media buatan ABSTRACT Artificial medium for in vitro culture is an alternative media to subtitute Murashige and Skoog (MS). Growmore added with organic substances as coconut water can be used as artificial medium. Coconut water can affect plant growth, so the research is to investigate the influence of coconut water in different concentration to chrysanth (Dendranthema grandiflora)’s growth should be done. The research was conducted in Laboratory of Molecular and Cell Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, Syiah Kuala University Darussalam, Banda Aceh, from April until July 2013. The research was designed by using Randomized Block Design with sub sampling. Media act as group, while coconut water concentration as treatment. Observation was started from growing time up to a month after planting. The result showed that there was no significant different between using MS and artificial medium with different coconut water concentration to initiate shootlet and rootlet, as well as the leaf growth. For chrysant hight, treatment K1 (2.5% of coconut water in medium) was different significantly from K0 (0% of coconut water in medium). The best growth of chrysant’s root presented in medium A, that was 4.8125 cm. Furthermore, the best growth of planted was in medium A as well, but it was not significantly different from one in Medium D (Growmore 1 gram/L), that is 9.1750 cm. Key words: Dendranthema grandiflora, coconut water, MS medium, artificial medium