ABSTRAK Resistensi bakteri terhadap antibiotik mendorong dilakukannya penelitian untuk menemukan alternatif bahan antibakteri yang berasal dari alam. Kecubung (Datura metel L.) merupakan salah satu tumbuhan obat tradisional yang telah terbukti memiliki aktivitas antibakteri. Penelitian ini menguji aktivitas antibakteri ekstrak n-heksana, etil asetat, dan metanol daun kecubung terhadap bakteri Methicillin Resisitant Staphylococcus aureus (MRSA). Ekstraksi daun kecubung dilakukan dengan metode maserasi bertingkat. Terhadap ekstrak n-heksana, etil asetat, dan metanol daun kecubung dilakukan skrining fitokimia, karakterisasi, dan uji aktivitas antibakteri. Karakterisasi ekstrak meliputi penentuan kadar air, kadar sari larut air dan etanol, serta kadar abu total. Uji aktivitas antibakteri ekstrak daun kecubung terhadap MRSA dilakukan menggunakan metode Kirby-Bauer dengan variasi konsentrasi ekstrak yaitu 5, 10, 15, dan 20%. Hasil skrining fitokimia terhadap ekstrak n-heksana menunjukkan adanya senyawa steroid. Ekstrak etil asetat menunjukkan adanya senyawa akaloid, dan steroid. Pada ekstrak metanol terdapat senyawa alkaloid, flavonoid, tanin, saponin, dan triterpenoid. Hasil karakterisasi terhadap ekstrak n-heksana menunjukkan kadar air sebesar 20,71%, kadar sari larut air dan etanol masing-masing 0,99% dan 40,72%, serta kadar abu total sebesar 2,78%. Ekstrak etil asetat menunjukkan kadar air sebesar 19,39%, kadar sari larut air dan etanol masing-masing 6,93% dan 73,88%, serta kadar abu total 2,86%. Pada ekstrak metanol didapatkan kadar air sebesar 16,42%, kadar sari larut air 14,51%, kadar sari larut etanol 85,02%, serta kadar abu total 3,62%. Pengujian aktivitas antibakteri menunjukkan hasil yang beragam untuk ketiga ekstrak. Ekstrak n-heksana tidak memiliki aktivitas antibakteri terhadap MRSA. Ekstrak etil asetat dan metanol daun kecubung memperlihatkan aktivitas antibakteri yang ditandai dengan terbentuknya zona hambat di sekitar kertas cakram. Masing-masing ekstrak menunjukkan aktivitas penghambatan terbesar pada konsentrasi 20% dengan rata-rata diameter zona hambat 15,85 mm dan 17,20 mm. Kata kunci: Daun kecubung (Datura metel L.), Methicillin Resistant Staphylococcus aureus (MRSA), dan metode Kirby-Bauer. ABSTRACT Resistance of bacteria to antibiotics had lead researchers to find new antibacterial material alternatives that comes from nature. Kecubung (Datura metel L.) which is traditional medicine that had provenforantibacterial activity. This study was examined antibacterial activity of n-heksane, ethyl acetate, and methanol extract of kecubung on Methicillin Resistant Staphylococcus aureus (MRSA). Extraction of kecubung leaves was done by graded maceration method. Phytochemical screening, characterization and antibacterial activity test was done on n-heksane, ethyl acetate, and methanol extract of kecubung leaves. Extract characterization included determination of water content, determination of water and ethanol soluble extracted content, and total ash content. Antibacterial activity test of kecubung leaves extract on MRSA was done by Kirby-Bauer method with extract concentration variation 5, 10, 15 and 20%. The presence of steroid had shown on the result of phytochemical screening on n-hexane extract. Ethyl acetic extract had shown the presence of alkaloid and steroid. The presence of alkaloid, flavonoid, tannin, saponin and triterpenoid had shown on phytochemical screening of n-hexane extract. The result of n-hexane characterization had shown that the water content is 20,71%, water and ethanol soluble extracted content 0,99% and 40,72% respectively, and total ash content was 2,78%. Ethyl acetate extract had shown the water content of extract is 19,39%, water and ethanol soluble extract content 6,93% and 73,88% respectively and total ash content as much as 2,86%. On methanol extract characterization had shown that the water content is 16,42%, water soluble extracted content 14,51%, ethanol soluble extract content 85,02% and total ash content was 3,62%. Antibacterial activity test had shown varied result for each extract. N-hexane extract was not show the presence of antibacterial activity on MRSA. Ethyl acetate and methanol extract of kecubung leaves had shown activity of antibacterial by forming inhibition zone around the disk. Each of the extract had shown the highest activity of inhibition at concentration of 20% with average diameter of inhibition zone 15,85 mm and 17,20 mm. Keywords: Kecubung leaves (Datura metel L.), Methicillin Resistant Staphylococcus aureus (MRSA), and Kirby-Bauer method.