ABSTRACT Testicular torsion is defined as the process of a testicular emergency as it causes cessation of testicular blood flow due to the twisting of spermatic cord. The research was subjected to know the effect of testicular torsion to Leydig cells include on the amount and the morphology of Leydig cells on ipsilateral Rattus norvegicus testes. This experimental research used completely randomized design (CRD) and divided the sample into 3 groups: control (K0) and experimental groups (P1 and P2) that were induced by 360° testicular torsion for 4 hours. P1 was examined after testicular torsion induced (short term effect) and P2 was examined after 30 days testicular torsion repaired (Long-term effect). Data were analyzed by using ANOVA one-way followed by Tukey HSD test. The result showed significant differences (P