ABSTRAK Formulasi dan uji aktivitas deodoran spray ekstrak etanol herba patikan kebo (Euphorbia hirta L.) telah dilakukan terhadap pertumbuhan Staphylococcus epidermidis secara in vitro. Penelitian ini bertujuan untuk menentukan stabilitas dan aktivitas sediaan deodoran spray ekstrak etanol herba patikan kebo terhadap pertumbuhan bakteri Staphylococcus epidermidis. Ekstraksi herba patikan kebo menggunakan metode maserasi dengan pelarut etanol 70%. Hasil karakterisasi ektrak menunjukkan bahwa persentase kadar air ekstrak 23% ± 1.633%; kadar sari larut air 72.33% ± 0.471%; kadar sari larut etanol 24.67% ± 0.9437%; dan kadar abu total 12,55% ± 0,304%. Hasil skrining fitokimia menunjukkan ekstrak etanol herba patikan kebo mengandung senyawa golongan alkaloid, tanin, flavonoid, dan steroid. Ekstrak etanol herba patikan kebo diformulasikan dalam sediaan deodoran spray dengan konsentrasi 10%. Deodoran spray tanpa penambahan ekstrak etanol herba patikan kebo digunakan sebagai kontrol negatif, sedangkan deodoran roll on Bellagio® Homme digunakan sebagai kontrol positif. Pengujian aktivitas antibakteri menggunakan metode difusi cakram. Sediaan deodoran spray ekstrak etanol herba patikan kebo diuji stabilitas fisik, keamanan dan aktivitasnya. Stabilitas fisik deodoran spray menunjukkan terjadi penurunan stabilitas pada siklus ke-6 dan penurunan pH hampir disetiap siklusnya. Hasil pengujian keamanan menunjukkan bahwa deodoran spray ekstrak etanol herba patikan kebo aman digunakan karena tidak mengiritasi kulit serta tidak merusak kain katun dan rayon. Hasil uji aktivitas terhadap bakteri Staphylococcus epidermidis menunjukkan aktivitas yang termasuk katergori kuat dengan nilai sebesar 11,742 mm ± 0,113 mm. Kata Kunci: Herba patikan kebo, ekstrak etanol, deodoran spray, Staphylococcus epidermidis. ABSTRACT Formulations and activity test of the ethanol extract herba deodorant spray patikan kebo (Euphorbia hirta L.) has been done against Staphylococcus epidermidis bacterial growth in vitro. This research to determine the stability in ethanol extract of herba spray deodorant formulations from patikan kebo as well as to verify the antibacterial activity against bacteria Staphylococcus epidermidis. The extraction of patikan kebo herba was carried out through maceration with ethanol 70%. The ethanol extract characterization’s results of the patikan kebo herb showed that the percentage of water content extract was 23% ± 1.633%; the percentage of water soluble content extract was 72.33% ± 0.0.471%; the percentage of ethanol soluble content extract was 24.67% ± 0.943%; and the percentage of total ash content was 12.55% ± 0.304%. The results from the phytochemical screening indicated that the ethanol extract consisted of alkaloids, tannins, flavonoids and steroids. Patikan kebo herba ethanol extract was formulated as deodorant spray with concentration of 10%. Deodorant spray without the addition of patikan kebo ethanol extract was used as negative control, while roll on deodoran Bellagio® Homme was used as positive control. The physical properties and safety of antibacterial deodorant spray formulation were tested and the antibacterial activity of the deodorant was conducted using the disc diffusion method. The study of the physical stability of the deodorant spray indicated a decline in stability on the 6th cycle as well as pH in every cycle. The safety test results showed that ethanol extract herba from patikan kebo was safe to be used both in term of irritation or demage to the fabric. The results of antibacterial test showed that the spray deodorant of ethanol extract from patikan kebo herba inhibition against Staphylococcus epidermidis was 11,742 mm ± 0,113 mm. Keywords: Patikan kebo herba, spray deodorant, Staphylococcus epidermidis.