Mineral water is primary need of living organism. Human’s body required 7 liters a day of water to maintained metabolism process. Mineral water control is very important to do to prevent bacteria invasion in mineral water. This study has a purpose to identified invasion of coliform bacteria in mineral water that sold at Syiah Kuala area, Banda Aceh. This study was held in Chemical Laboratory, Math and Sciences Faculty of Syiah Kuala University on April-May 2012, using 17 samples of mineral water that collected from mineral water selling depot in Syiah Kuala area. The samples were taken using systematic random sampling method with slovin formula and mean 0,1. Sample’s test including persumtive test and followed with confirmed test and then data were analyzed and showed in MPN table. The result show that MPN number for all 17 samples that checked were 0 bacteria/mL water. Based on that result we can conclude that mineral water in Syiah Kuala area were uncontaminated by coliform bacteria. It requires a continuously test and control to prevent invasion of coliform on mineral water. Keywords : mineral water, coliform