ABSTRACT This research aimed to determine the effect of flower size and concentration of picloram, as well as determine the effect of interaction flower size and concentrations of picloram in induced somatic embryos Theobroma cacao L. adaptive northern Aceh. This research was conducted at the Tissue Culture Laboratory, Faculty of Agriculture, Syiah Kuala University, using a completely randomized design in 3x5 factorial within five replications.The first factor was flower size consisting of 2, 4 and 6 mm as well as the second factor was concentration of picloram consisting of 2, 2.5, 3, 3.5, and 4 mg / l. Data were analyzed using ANOVA and mean comparison test using test DNMRT treatment at the level of 5%. The results showed that the average explants forming somatic embryos reached 60% with the highest average number of embryos per explant of 1.3 embryos on flower size 6 mm. Average explants forming callus and embryogenic callus explant respectively reached 77% and 44% on 2 mg / l concentration picloram. Combination of flower size 6 mm with 2 mg / l concentration of picloram forming an average of the highest embryogenic callus reached 72%. Key words: Theobroma cacao L., Picloram, Somatic Embryos, Embryogenic callus, Callus, Explant