ABSTRAK Penelitian ini bertujuan untuk melihat pengaruh penambahan ampas tebu (Saccharum officinarum) pada substrat bibit jamur merang (Volvariella volvacea) terhadap produksi bibit jamur merang. Penelitian telah dilaksanakan di Laboratorium Biologi Sel Molekuler dan Laboratorium Mikrobiologi Jurusan Biologi FMIPA Unsyiah dan di tempat budidaya jamur merang ”Lee Guna” jln. Teuku Nyak Arif, Lamnyoeng Banda Aceh. Penelitian dilaksanakan dari Agustus 2013 sampai Januari 2014. Penelitian ini dilakukan secara deskriptif dengan metode Rancangan Acak Kelompok, dengan 4 perlakuan dan 6 kali ulangan. Parameter yang diamati kecepatan pertumbuhan miselium, awal dan lamanya panen, berat basah tubuh buah total, diameter tubuh buah, berat rata-rata per tubuh buah dan Rasio Efisiensi Biologis (REB). Data dianalisis menggunakan analisis varian (ANAVA) kemudian dilanjutkan dengan uji BNJ. Hasil penelitian menunjukkan bahwa kecepatan pertumbuhan miselium jamur merang pada media bibit yang ditambahkan ampas tebu terdapat perbedaan. Perlakuan M0 (kontrol) mengalami pertumbuhan lebih cepat dan diikuti perlakuan M1, sedangkan perlakuan M2 dan M3 relatif lebih lambat. Panen awal pada produksi jamur untuk tiap perlakuan berbeda. Penambahan ampas tebu pada bibit mempengaruhi diameter dan berat total tubuh buah jamur yang dihasilkan, namun tidak berpengaruh nyata pada berat rata-rata per tubuh buah jamur yang dihasilkan. Rasio efisiensi biologis jamur merang menggunakan tandan sawit ternyata kurang efisien. Kata kunci : Jamur merang (Volvariella volvacea), komposisi media bibit, ampas tebu ABSTRACT The research aim to know about the effect of sugar waste cane (Saccharum officinarum) addition on merang mushroom (Volvariella volvacea) spawn to fruit body production. The research conducted at Biological Cell of Molekuler Laboratory and Laboratory of Microbiology Department of Biology FMIPA Unsyiah and the mushroom nursery "Lee Guna" at jln. Teuku Nyak Arif, Lamnyoeng Banda Aceh, from August 2013 to January 2014. Descriptive research is performed using randomized block design with 4 treatments and six repetition. The observed parameters were mycelium growth rate, the time required for the first body emerged and harvest, the total wet weight of fruiting body, diameter of fruiting body, the weight of the fruiting bodies obtained from each harvested, Biological Efficiency Ratio (BER). Data were analyzed using analysis of variance (ANAVA) then followed by BNJ test. The results showed that the growth rate of mushroom mycelium on the spawn medium that were added by bagasse there is a difference. M0 (control) experienced faster growth and followed by treatment of M1, M2 while treatment and M3 is relatively slower. Harvest early in mushroom production for each different treatment. Addition of bagasse in influencing spawn diameter and weight of the total mushroom fruiting body is produced, but had no significant effect on the average weight per fruiting body produced by fungi. Biological efficiency ratio mushroom was less efficient oil palm bunches. Key words: Merang mushroom (Volvariella volvacea), spawn composition, sugar cane