ABSTRAK Methicillin resistant Staphylococcus aureus (MRSA) merupakan galur bakteri S. aureus yang resisten terhadap antibiotik jenis betalaktam, termasuk penisilin dan turunannya. Paramedis yang carrier MRSA dikhawatirkan dapat menjadi penyebab penularan ke pasien terutama di ruang intensif. Penelitian ini bertujuan untuk melakukan isolasi dan identifikasi MRSA dari mukosa hidung paramedis di ruang intensif RSUD dr. Zainoel Abidin Banda Aceh. Pengambilan sampel dilakukan terhadap paramedis yang bertugas di ruang intensive care unit (ICUD), intensive coronary care unit (ICCU), neonate intensive care unit (NICU), paediatric intensive care unit (PICU) dan intensive care unit (ICU) jantung. Total sampel yang diambil ialah 38 sampel mukosa hidung paramedis. Identifikasi bakteri meliputi identifikasi makrokopis, pewarnaan Gram, identifikasi mikroskopis, uji katalase, dan uji koagulase. Uji sensitifitas antibiotik dilakukan dengan menggunakan metode difusi cakram. Parameter yang diukur ialah jumlah sampel positif dan negatif MRSA serta perbandingan persentase positif pada tiap sampel di ruang yang berbeda. Total insidensi MRSA pada paramedis di ruang intensif RSUDZA ialah 26%. Hasil penelitian ini ialah angka insidensi infeksi MRSA di ruang ICUD, ICU jantung, ICCU, PICU, dan NICU masing-masing ialah 8,3%, 14,3%, 14,3%, 60%, dan 57,2%. Hasil penelitian ini menunjukkan bahwa insidensi MRSA pada paramedis di ruang intensif RSUDZA tergolong berbahaya karena dapat menjadi sumber penularan kepada pasien. Kata Kunci : MRSA, Staphylococcus aureus, infeksi nosokomial, paramedis, rumah sakit. ABSTRACT Methicillin resistant Staphylococcus aureus (MRSA) is a strain of S. aureus that has resistance on betalactam antibiotics, including penicillin and its derivate. MRSA-carrier paramedics were concerned to be the spreading cause to patients, particularly in intensive care rooms. This research was aimed to isolate and identify MRSA from nose mucous of paramedics in intensive rooms of dr. Zainoel Abidin Regional Public Hospital (RSUD), Banda Aceh. Samples were collected from on duty paramedics in Intensive Care Unit (ICU), Intensive Cardiac Care Unit (ICCU), Neonatal Intensive Care Unit (NICU), Pediatric Intensive Care Unit (PICU), and Heart ICU rooms. Bacteria were identified using macroscopic identification, Gram-staining, microscopic identification, and advanced tests including catalase, coagulase, and antibiotic-sensitivity tests (using disc diffusion method). Parameters observed were number of positive- and negative-MRSA samples, as well as positive percentage comparison in different rooms. Total incidence was 26% positive-MRSA. The result showed that MRSA incidence were each 8,3% in ICU, 14,3% in Heart ICU, 14,3% in ICCU, 60% in PICU and 57,2% in NICU. This results indicated that MRSA incidence on paramedics in intensive rooms of RSUDZA was categorized as hazardous due to its possibility to be the spreading cause to patients. Keywords: MRSA, Staphylococcus aureus, nosocomial infection, paramedic, hospital.