ABSTRAK Penggunaan abate dalam pemberantasan larva Aedes aegypti dapat memicu terjadinya resistensi. Salah satu tumbuhan yang dapat dikembangkan sebagai larvasida adalah daun tapak dara (Catharantus roseus (L.) G. Don). Penelitian terhadap daun tapak dara merupakan usaha untuk mendapatkan jenis larvasida hayati. Tujuan penelitan ini adalah untuk mengetahui efek larvasida ekstrak metanol daun tapak dara terhadap larva Aedes aegypti instar III. Penelitian ini dilakukan di laboratorium Parasitologi Fakultas Kedokteran Universitas Syiah Kuala, pada bulan Maret 2015. Metode Penelitian Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 4 pengulangan. Analisis data yang digunakan adalah analisa probit (LC50). Kesimpulan hasil uji larvasida ekstrak metanol daun tapak dara secara analisis probit memberi nilai LC50 adalah 1001,789 ppm. Berdasarkan dari hasil LC50 ekstrak metanol daun tapak dara dapat berpengaruh terhadap larva Aedes aegypti instar III. Sifat larvasida dari ekstrak metanol daun tapak dara mengandung senyawa metabolit sekunder berupa alkaloid, saponin dan flavanoid. Senyawa tersebut dapat masuk ke dalam tubuh larva sehingga menyebabkan sistem pertahanan hidup larva Aedes aegypti instar III terganggu. Kata Kunci : Daun tapak dara, Larvasida, Aedes aegypti ABSTRACT The use of abate for eraducation of Aedes aegypty larvae can lead to resistence. One of the plants that can be developed as larvicide is tapak dara leaf (Catharantus roseus (L.) G. Don). The reasearch of tapak dara leaves is a business for finding a type of bio larvicide. The objektive of this research was to find out the effect of larvicide of methanol extract from tapak dara leaves towards Aedes aegypti larvae of instar III. This research was conducted in the Parasitologi Laboratory of Medical Faculty in Syiah kuala University, In March 2015. The method of research was completely randomized design with 6 treatments and 4 repetitions. Data analysis used was probit analysis (LC50). The conclusion of test results of methanol extract from tapak dara leaves by using the probit analysis showing a value of LC50 was 1001.789 ppm. Based on the result of LC50, methanol extract from tapak dara leaves can be influenced on Aedes aegypti instar III. The larvicidal characteristic of methanol extract from tapak dara leaves is that it contains secondary metabolite compounds, such as alkaloids, saponins, and flavanoids. Those compounds can enter into the body of larvae so that they can cause a life defense system of Aedes aegypti of instar III to be affected. Key words : Tapak dara leaves, Larvicide, Aedes aegypti