ABSTRAK Binahong (Anredera cordifolia (Ten.) Steenis) merupakan suatu tanaman obat tradisional yang dapat menangani berbagai jenis penyakit termasuk sebagai penurun glukosa darah. Penelitian ini bertujuan untuk mengetahui efikasi ekstrak etanol daun binahong terhadap penurunan kadar gula darah pada tikus putih. Penelitian ini merupakan penelitian eksperimental laboratorik menggunakan rancangan pretest postest dengan kelompok kontrol (pretest posttest control group design). Sampel yang digunakan adalah tikus putih jantan strain wistar sebanyak 25 ekor dibagi dalam 5 kelompok yaitu, kelompok kontrol negatif, kelompok kontrol positif dan kelompok perlakuan dosis 50 mg/kgBB, dosis 100 mg/kgBB dan dosis 200 mg/kgBB. Peningkatan kadar gula darah hewan uji dilakukan dengan larutan glukosa 10%, setelah itu dilakukan pemeriksaan kadar gula darah pada menit 30 , menit 60, menit 90, menit 120 dan menit 150 setelah induksi glukosa. Hasil kadar glukosa dianalisis mengggunakan uji Kruskall-Wallis dan dilanjutkan dengan uji posthoc Mann-Whitney. Kesimpulan dari penelitian ini adalah ekstrak etanol daun binahong dapat menurunkan kadar gula darah pada tikus putih jantan strain wistar yang diinduksi dengan glukosa. Kata Kunci : Binahong (Anredera cordifolia (Ten.) Steenis) , Hiperglikemia, ABSTRACT Binahong (Anredera cordifolia (Ten) Steenis) is one of the traditional medicine used to traet several diseases including decreasing blood glucose level. The aim of this research was to know effication extrack ethanol of binahong heartleaf to decreasing blood glucose level on rat. This research was an experimental research on experimental animals with pretest posttest control group design. The sample of this research is 25 male rat (Rattus norvegicus) divide randomly into 5 group of treatment and all of the group was given oral glucose tolerance, 3 groups used toheartleaf binahong extract with the dose of 50 mg/kgBB, 100 mg/kgBB and 200 mg/kgBB, a group was given acarbose as positive control and a group was given aquades as negative control. All of the rat was given oral glucose 10% to increasing blood glucose level. In order to examine the response, blood glucose levels were observed in 0 minute, 30 minute, 60minute, 90 minute, 120 minute, 150 minute, after being induced by glucose. The result of blood glucose tolerance level was analyzed with Kruskall Wallis and it was continued by post hoc Mann-whitney test. The conclusion of this research is there was an effect heartleaf of binahong to decreasing blood glucose level in rat. Key word: Binahong Anredera cordifolia (Ten) Steenis, Hyperglicemic