ABSTRACT Euphorbia hirta has been known for medicine properties. Euphorbia hirta contains secondary methabolites such as flavonoids, tannins, and alkaloids that have antibacterial ability. This experiment was conducted to determine the antibacterial extract of Euphorbia hirta based on the growth inhibition of MRSA. This study used the Complete Randomized Design (CRD) with six treatment and five repetitions. The treatment consist of Euphorbia hirta extract with a concentration of 20%, 40%, 60%, 80%, and two in the controls of vancomycin as a positive control and distilled water as a negative control. The parameter that is measured is the inhibition area on the media. Data were analyzed by Analysis of Variance (ANOVA) and followed by Honestly Significance Defference test (HSD). Euphorbia hirta extract with a concentration of 20%, 40%, 60%, and 80% result the inhibition area with average diameter of 12,4 mm, 15,2 mm, 16,5 mm and 15,3 mm. ANOVA test at level α = 0,05 indicates that the Euphorbia hirta extract showed significant differences in inhibiting the growth of MRSA. Keyword : Euphorbia hirta L. (patikan kerbau), inhibition zone, Methicillin Resistant Staphylococcus aureus (MRSA)