ABSTRAK Alkaloid benzylisoquinoline dan aporphine telah berhasil diisolasi dari kulit batang Dehaasia longipetiolata. Maserasi digunakan untuk proses ekstraksi dan kromatografi kolom digunakan untuk proses isolasi komponen alkaloid yang ada dalam sampel kulit batang D. longipetiolata. Ekstrak pekat alkaloid diisolasi menggunakan kromatografi kolom dengan fasa diam silika gel 60 dan fasa gerak etil asetat : metanol (100:0; 9,5:0,5; 9:1; dan 8:2) dengan sistem gradien elusi. Struktur senyawa alkaloid diidentifikasi menggunakan metode spektroskopi 1D dan 2D-NMR. Kata kunci : Dehaasia longipetiolata, Lauraceae, benzylisoquinoline, aporphine. ABSTRACT Benzylisoquinoline and aporphine alkaloids have been isolated from stem bark of Dehaasia longipetiolata. Maceration was used for extraction and column chromatography was used for separation of the compounds from stem bark of D. longipetiolata. Crude extract alkaloids were separated by using column chromatography with silica gel G-60 as stationary phase. Ethyl acetate and methanol were used as solvent system in column chromatography. The structures of isolated alkaloids were determined on the basis of 1D and 2D NMR spectroscopic data. Keywords: Dehaasia longipetiolata , Lauraceae, benzylisoquinoline, aporphine.