ABSTRAK Penelitian ini bertujuan untuk mengetahui kandungan fitokimia daun tumbuhan kapas (Gossypium hirsutum L.) dan mengukur daya hambat ekstrak n-heksana dan metanol daun tumbuhan G. hirsutum terhadap bakteri Methicillin Resistant Staphylococcus aureus (MRSA). Sampel daun tumbuhan kapas diperoleh dari desa Lamlhom, Aceh Besar. Ekstraksi dan uji fitokimia dilakukan di Laboratorium Penelitian Jurusan Kimia. Uji antibakteri dilakukan di Laboratorium Mikrobiologi Jurusan Biologi FMIPA Universitas Syiah Kuala. Pengujian antibakteri menggunakan metode Rancangan Acak Lengkap (RAL) dengan 5 perlakuan, yaitu kontrol negatif (pelarut), ekstrak uji dengan konsentrasi masing-masing 20%, 30%, 40% dan kontrol positif (linezolid 30 µg) yang diulangi sebanyak tiga kali. Hasil uji fitokimia diketahui bahwa sampel segar daun G. hirsutum mengandung senyawa alkaloid, steroid, terpenoid, dan flavonoid. Ekstrak n-heksana mengandung steroid dan terpenoid, sedangkan ekstrak metanol mengandung alkaloid, terpenoid, dan flavonoid. Hasil uji antibakteri didapatkan bahwa ekstrak n-heksana dan metanol daun G. hirsutum pada konsentrasi 40% memberi daya hambat paling baik terhadap MRSA dibandingkan ekstrak dengan konsentrasi 20% dan 30%. Kata Kunci: Antibakteri, Gossypium hirsutum L., Methicillin Resistant Staphylococcus aureus (MRSA), fitokimia. ABSTRACT This research was carried out to know the phytochemical constituents of cotton leaves (Gossypium hirsutum L.) and to measure the inhibition zone of hexane and methanol extract of G. hirsutum leaves against Methicillin Resistant Staphylococcus aureus (MRSA) bacteria. The leaves of cotton obtained at Lamlhom Village, Great Aceh. Extraction and phytochemical test conducted at Research Laboratory of Chemistry Department. Antibacterial test conducted at Microbiology Laboratory of Biology Department, Mathematic and Natural Science, Syiah Kuala University. The antibacterial test uses Completely Randomized Design (CRD) with 5 treatments, there are negative control (solvent), with a test extract concentrations respectively 20%, 30%, 40% and positive control (linezolid 30 µg) is repeated three time. The phytochemical test showed that fresh sample of cotton leaves contains alcaloid, steroid, terpenoid, and flavonoid compounds. Hexane extract contains steroid and terpenoid compounds. Methanol extract contains alcaloid, terpenoid, and flavonoid compounds. The result of antibacterial showed that hexane and methanol extract in 40% gives the better inhibition zone against MRSA than methanol extract in 20% and 30%. Keyword: Antibacterial, Gossypium hirsutum L., Methicillin Resistant Staphylococcus aureus (MRSA), phytochemical