Abstrak

Baca Juga : UJI KETAHANAN BAKTERIOSIN PRODUKSI BAKTERI ASAM LAKTAT USUS AYAM PETELUR YANG DIBERI AKBISPROB TERHADAP SUHU DAN PH (LUTHFI PHONNA, 2018) ,

Baca Juga : EFEK PENAMBAHAN CACO3 TERHADAP PERTUMBUHAN DAN AKTIVITAS ANTIBAKTERI BAKTERI ASAM LAKTAT (BAL) ASAL USUS AYAM PETELUR YANG DIBERI AKBISPROB (Nailah Musni, 2019) ,

silkan aktivitas yang tergolong aktif kuat (>5 mm) pada suhu 120°c selama 45 menit. isolat bal p1h2k1, p2h3k1, p3s3k2, p3h2k1, p3h1k1, p3s3k1, dan p3s1k1 memiliki aktivitas tergolong aktif lemah (0,5-2,4 mm) setelah pemanasan 120°c selama 45 menit. isolat p2s1k1, p3s3k3, dan p3h1k4 tidak stabil pada pemanasan 120°c. pada uji ketahanan terhadap ph semua supernatan dari isolat bal memiliki aktivitas antibakteri pada rentang ph 2, 4, dan 6. berdasarkan hasil penelitian menunjukkan 13 isolat bal mampu memproduksi bakteriosin produksi bal usus ayam petelur yang diberi akbisprob 4% tahan panas dan stabil ph 2 sampai 6. perlakuan pemberian akbisprob 4% (lima hari sekali) menghasilkan tiga isolat penghasil bakteriosin (supernatan bebas sel) yang tahan suhu 120°c selama 45 menit dan empat isolat yang tahan pada rentang ph 2 sampai 6. resistance test of bacteriocins from lactic acid bacteria isolated from intestine laying hens fed with akbisprob on temperature and ph abstract this study aim to determine the resistance of bacteriocin (supernatant) lab isolated from the laying hens intestine after fed akbisprob on temperature and ph. lactic acid bacteria were obtained from previous reserch isolated from the small intestine and caecum laying hens after fed of akbisprob. the research was using kirby-bauer or disk diffusion method. the temperature divided into six treatments 70, 80, 90, 100, 110 and 120°c and heated for 15, 30, 45 minutes. the treatment of ph divide in 2, 4, and 6. lactic acid bacteria supernatant was obtained by culturing on mrsb and then centrifuge at 13,000 rpm for 10 minutes at 4°c. bacteriocin (lab supernatant) tested for antibacterial activity against bacillus subtilis. the resistance to temperature test was obtained on supernatant of p3h1k3, p3h1k2 and p3s1k2 activities that were strogly active (>5 mm) at 120°c for 45 minutes. the supernatant isolates p1h2k1, p2h3k1, p3s3k2, p3h2k1, p3h1k1, p3s3k1, and p3s1k1 had antibacterial activity at 120°c for 45 minutes but the activity was low active (0,5- 2,4 mm). lactic acid bacteria supernatant free cell (bacteriocin) isolate p2s1k1, p3s3k3, and p3h1k4 had unstable at 120°c. in the ph resistance test all supernatant from lab isolates had antibacterial activity at ph 2, 4, and 6. based on the result of research, it can be concluded bacteriocin produce lab from laying hens intestine after fed akbisprob 4% (five days once) were able produce three isolates bacteriocin thermostable at 120°c for 45 minutes and four isolates active over a wide ph range of 2-6. ?

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