Abstrak

Baca Juga : PERUBAHAN PH SALIVA SEBELUM DAN SESUDAH MENGKONSUMSI BUAH PISANG AYAM (MUSA ACUMINATA COLLA) PADA MAHASISWA FKG UNSYIAH ANGKATAN 2014 (NURA SHARA AMIRZA, 2017) ,

Baca Juga : PENGGUNAAN ZAT PENGATUR TUMBUH BENZIL AMINO PURIN (BAP) DAN NAPHTHALENE ACETIC ACID (NAA) TERHADAP INDUKSI TUNAS PISANG BARANGAN (MUSA ACUMINATA COLLA.) SECARA IN VITRO (WIKA RAHMATIKA, 2017) ,

p (ral) dengan 4 perlakuan dan 5 kali ulangan. perlakuan ini terdiri atas penambahan 2,4-d dengan konsentrasi: 0 ppm (kontrol); 1 ppm; 2 ppm; 3 ppm dan 4 ppm ke dalam media murashige and skoog with vitamins (ms) + 2 ppm bap. parameter yang diamati adalah jumlah eksplan yang menumbuhkan kalus dan waktu kemunculan kalus. hasil penelitian menunjukkan bahwa perlakuan 2 ppm 2,4-d + 2 ppm bap adalah konsentrasi yang tepat untuk menumbuhkan kalus pisang barangan dengan persentase ekpslan yang menumbuhkan kalus sebanyak 36%. kata kunci : musa acuminata colla, kalus, eksplan, media murashige and skoog with vitamins, 2,4-d, bap. abstract the multiplication of quality barangan banana seedlings (musa acuminata colla) can be done with modern technology, one of which is callus tissue culture. callus is a group of cells that develops and has not been differentiated. callus induction is more beneficial in seedligs multiplication, because callus can be cultured continuously. explants from ovaries have a lower risk of contamination in the culture process. callus culture requires plant grow regulators (pgrs) with the right concentration. this research is about the influence of 2,4-d on the formation of barangan banana (musa acuminata colla) callus from the ovary. the research was conducted on july until october 2018 in tissue culture laboratories, field of horticulture, aceh agriculture department. the purpose of the research is to get the suitable concentration of 2,4-d to combined with 2 ppm bap which can induce calluses of barangan bananas from the ovary. the research used a completely randomized design (crd) with 4 treatments and 5 times repetition. this treatment consisted of adding 2,4-d with a concentration: 0 ppm (control); 1 ppm; 2 ppm; 3 ppm and 4 ppm into murashige and skoog media with vitamins (ms) + 2 ppm bap. the parameters observed were the number of explants which induced callus and the time of callus appearance. the results showed that the treatment of 2 ppm 2,4-d + 2 ppm bap was the suitable concentration resulting 36% callus were induced. keywords: musa acuminata colla, callus, explant, murashige media and skoog with vitamins, 2,4-d,

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