Abstrak

Baca Juga : PRODUKSI POLIHIDROKSIALKANOAT (PHA) DARI BACILLUS SUBTILIS DENGAN MENGGUNAKAN MEDIA MINYAK PLIEK U (Muna Safitri, 2020) ,

Baca Juga : PRARANCANGAN PABRIK REGENERASI MINYAK JELANTAH DENGAN KAPASITAS PRODUKSI 87.000 TON/TAHUN (Ridwansyah, 2014) ,

nunjukkan adanya granula pha di dalam sel bacillus subtilis. hasil karakterisasi menggunakan fourier transform infra-red (ftir) memberikan puncak khas pha yaitu dengan gugus fungsi o-h, c-h, c=o, ch3, c-o-c dan c-o pada bilangan gelombang 3439 cm-1, 2930 cm-1, 1730 cm-1, 1453 cm-1, 1276 cm-1, 1055 cm-1 secara berturut-turut. hasil karakterisasi menggunakan x-ray difraction (xrd) menunjukkan struktur amorf dan kristalinitas rendah dengan rata-rata ukuran kristal sebesar 42,87 nm dan karakterisasi morfologi permukaan menggunakan scanning electron microscopy (sem) menunjukkan permukaan yang berserat. karakterisasi termal menggunakan differential scanning calorimeter (dsc) dan thermal gravimetric analysis (tga) menunjukkan sifat termal khas pha dengan titik leleh (tm) pada suhu 163,54 ºc dan titik degradasi (td) pada suhu 311 ºc . kata kunci : minyak jelantah, bacillus subtilis, polihidroksialkanoat (pha). abstract polyhydroxyalkanoate (pha) is a type of biopolymer produced by bacteria as a secondary metabolite. the production of pha by bacteria occurs in a state of nutrient imbalance and excess carbon. as a result, the production of pha from bacteria requires a large cost to obtain a carbon source. the best step that can be done is to use wasted as a source of carbon. one of wasted that can be a source of carbon is wasted cooking oil. this research has succeeded in producing pha from bacillus subtilis using wasted cooking oil as a carbon source. pha production from bacillus subtilis was carried out in a modified mineral salt medium (msm) by adding 2% wasted cooking oil and 0.1% yeast extract. cell biomass was obtained after 72 hours of growth at 30 ºc and shaking 150 rpm. pha isolation was carried out by chloroform extraction method and precipitation with cold methanol. the results of staining with sudan black showed the presence of pha granules in bacillus subtilis cells. the results of characterization using fourier transform infra-red (ftir) give a typical peak of pha, namely the o-h, c-h, c = o, ch3, c-o-c and c-o groups at wave numbers 3439 cm-1, 2930 cm-1, 1730 cm-1, 1453 cm-1, 1276 cm-1, 1055 cm-1 respectively. the results of characterization using x-ray difraction (xrd) showed an amorphous structure and low crystallinity with an average crystal size of 42.87 nm and surface morphological characterization using scanning electron microscopy (sem) showed the presence of fiber surface. thermal characterization using differential scanning calorimeter (dsc) and thermal gravimetric analysis (tga) shows typical thermal properties of pha with melting point (tm) at 163.54 ºc and degradation point (td) at 311 ºc. keywords : wasted cooking oil, bacillus subtilis, polyhydroxyalkanoate

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