Abstrak

Baca Juga : EFEK PERBEDAAN WAKTU PENGAMBILAN SAMPEL DARAH DAN FESES TERHADAP KOSENTRASI HORMON KORTISOL PADA SAPI ACEH (AHMAD WAHYUDI, 2018) ,

Baca Juga : PERBANDINGAN WAKTU INKUBASI UNTUK ANALISIS HORMON STRES SECARA NON-INVASIF MENGGUNAKAN METODE ENZYME LINKED IMMUNOSORBENT ASSAY (REZA SAPUTRA, 2019) ,

hormon kortisol di dalam plasma pada pagi hari secara signifikan lebih tinggi 79,34% dibandingkan siang hari (p0,05). kesimpulan, terdapat variasi diurnal hormon kortisol pada plasma sapi aceh pada pagi dan siang hari, tetapi pada sampel feses tidak terlihat variasi diurnal. xi the effect of different time of sample collection of blood and feces on the cortisol concentration in aceh cattle abstract one of the parameter that must be evaluated prior to perform the cortisol hormone analysis is time of sample collection. this is due to there is diurnal variation of cortisol concentrations in the morning and noon. therefore,this study aims to measure cortisol concentration in the blood and feces collected in the morning and noon for evaluating the diurnal variation in aceh cattle. samples used in this study were 16 blood and feces samples (8 samples in the morning and 8 samples in the noon) collected from eight adult females of aceh cattle age 2-3 years old. blood samples were prepared into a plasma, while the feces samples were extracted and then performed cortisol hormone measurement through enzyme-linked immunosorbent assay (elisa) technique. data were analyzed by using t-test. the result showed that concentrations of cortisol in the plasma collected in the morning were significantly higher 79,34% compared to the noon samples (p 0,05). in conclusion, there is a variation diurnal in plasma cortisol of aceh cattle, but there is no variation diurnal in feces cortisol of aceh cattle.

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