Most probable number and membrane filter were methods that can be used for analyzing of escherichia coli contained in refill drinking water. based on permenkes 492/menkes/per/iv/2010 about the regulations of drinking water quality, the content of escherichia coli in a 100 ml sample is zero. the research aimed to know the differences in the content of escherichia coli in refill drinking water which were analized by most probable number method and membrane filter method. the research used observation method with descriptive analytic design which is carried out on 28 samples of drinking water refill depot in city center and downtown area. the data were analyzed by t-test and anova using spss version 16. the results showed that there is a significant difference between the membrane filter method and most probable number method. anova test showed no significant difference between the downtown area and the city center either to the monitored or unmonitored region. the average